| 产品名称： | SK-UT-1B |
|---|---|
| 商品货号： | TS210888 |
| Organism： | Homo sapiens, human |
| Tissue： | uterus; endometrium |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma |
| Age： | 75 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Karyotype： | (P27) hypodiploid to diploid |
| Clinical Data： | 75 years Caucasian female |
| Antigen Expression： | Blood type B; Rh+ |
| Genes Expressed： | Blood type B; Rh+ |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | This is a substrain of SK-UT-1 (see ATCC HTB-114) which reportedly differs in morphology, karyology and in the type of tumor which develops on animal inoculation. The cells form well differentiated adenocarcinomas consistent with endometrial carcinoma. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 10,13 D16S539: 12,14 D5S818: 10,11 D7S820: 9,10 THO1: 7 TPOX: 8 vWA: 16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | G Trempe |
| Deposited As： | Homo sapiens |
| References： | Chen TR. SK-UT-1B, a human tumorigenic diploid cell line. Cancer Genet. Cytogenet. 33: 77-81, 1988. PubMed: 3383166 Chen TR. SK-UT-1B cell line has the diploid karyotype. A reply. Cancer Genet. Cytogenet. 48: 139-141, 1990. PubMed: 2372783 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 |
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| 产品名称： | SK-UT-1B |
|---|---|
| 商品货号： | TS210888 |
| Organism： | Homo sapiens, human |
| Tissue： | uterus; endometrium |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma |
| Age： | 75 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Karyotype： | (P27) hypodiploid to diploid |
| Clinical Data： | 75 years Caucasian female |
| Antigen Expression： | Blood type B; Rh+ |
| Genes Expressed： | Blood type B; Rh+ |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | This is a substrain of SK-UT-1 (see ATCC HTB-114) which reportedly differs in morphology, karyology and in the type of tumor which develops on animal inoculation. The cells form well differentiated adenocarcinomas consistent with endometrial carcinoma. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 10,13 D16S539: 12,14 D5S818: 10,11 D7S820: 9,10 THO1: 7 TPOX: 8 vWA: 16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | G Trempe |
| Deposited As： | Homo sapiens |
| References： | Chen TR. SK-UT-1B, a human tumorigenic diploid cell line. Cancer Genet. Cytogenet. 33: 77-81, 1988. PubMed: 3383166 Chen TR. SK-UT-1B cell line has the diploid karyotype. A reply. Cancer Genet. Cytogenet. 48: 139-141, 1990. PubMed: 2372783 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 |
