| 产品名称： | SK-UT-1 |
|---|---|
| 商品货号： | TS210893 |
| Organism： | Homo sapiens, human |
| Tissue： | uterus |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma |
| Age： | 75 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Karyotype： | (P8) hypodiploid to hyperdiploid |
| Clinical Data： | 75 years Caucasian female |
| Antigen Expression： | Blood Type B; Rh+ |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | The cells form spindle cell sarcomas. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for use with a 5% CO2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC® Catalog No. 30-2020 (500 mL) and ATCC® Catalog No. 30-2021 (100 mL).
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Medium Renewal: Twice per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 13,14 D5S818: 10,11 D7S820: 9,10 THO1: 7 TPOX: 8 vWA: 15,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B Me-2, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 |
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| 产品名称： | SK-UT-1 |
|---|---|
| 商品货号： | TS210893 |
| Organism： | Homo sapiens, human |
| Tissue： | uterus |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma |
| Age： | 75 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Karyotype： | (P8) hypodiploid to hyperdiploid |
| Clinical Data： | 75 years Caucasian female |
| Antigen Expression： | Blood Type B; Rh+ |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | The cells form spindle cell sarcomas. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for use with a 5% CO2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC® Catalog No. 30-2020 (500 mL) and ATCC® Catalog No. 30-2021 (100 mL).
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Medium Renewal: Twice per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 13,14 D5S818: 10,11 D7S820: 9,10 THO1: 7 TPOX: 8 vWA: 15,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B Me-2, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 |
