SK-PN-DW

规格:

货期:

编号:TS210894

品牌:Testobio

产品名称：	SK-PN-DW
商品货号：	TS210894
Organism：	Homo sapiens, human
Tissue：	retroperitoneal embryonal tumor
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	malignant primitive neuroectodermal tumor
Age：	17 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	aneuploid; modal number = 40 to 41; monosomy 13 was found in 100% of the cells; iso(17q) and monosomy 9, 10, 11, and 18 were found in greater than 20% of cells analyzed., In a large proportion of the cells, the double minutes per cell were too numerous to count.
Derivation：	SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor.
Clinical Data：	17 years male Caucasian
Genes Expressed：	Cholecystokinin (CCK)
Cellular Products：	Cholecystokinin (CCK)
Tumorigenic：	Yes
Effects：	Yes, forms tumors in nude mice
Comments：	The cells express CCK specific mRNA and synthesize considerable quantities of variably processed CCK prohormone.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: Twice per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 inCulture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 12 D13S317: 9 D16S539: 12 D5S818: 13,14 D7S820: 8,14 THO1: 6 TPOX: 8,9 vWA: 16
Name of Depositor：	C Helson
Deposited As：	Homo sapiens
Year of Origin：	1979
References：	Potluri VR, et al. Primitive neuroectodermal tumor cell lines: chromosomal analysis of five cases. Cancer Genet. Cytogenet. 24: 75-86, 1987. PubMed: 3024811 Schneider BS, et al. Expression of the cholecystokinin gene by cultured human primitive neuroepithelioma cell lines. J. Clin. Endocrinol. Metab. 69: 411-419, 1989. PubMed: 2753982 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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SK-PN-DW

货号: TS210894
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产品名称：	SK-PN-DW
商品货号：	TS210894
Organism：	Homo sapiens, human
Tissue：	retroperitoneal embryonal tumor
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	malignant primitive neuroectodermal tumor
Age：	17 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	aneuploid; modal number = 40 to 41; monosomy 13 was found in 100% of the cells; iso(17q) and monosomy 9, 10, 11, and 18 were found in greater than 20% of cells analyzed., In a large proportion of the cells, the double minutes per cell were too numerous to count.
Derivation：	SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor.
Clinical Data：	17 years male Caucasian
Genes Expressed：	Cholecystokinin (CCK)
Cellular Products：	Cholecystokinin (CCK)
Tumorigenic：	Yes
Effects：	Yes, forms tumors in nude mice
Comments：	The cells express CCK specific mRNA and synthesize considerable quantities of variably processed CCK prohormone.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: Twice per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 inCulture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 12 D13S317: 9 D16S539: 12 D5S818: 13,14 D7S820: 8,14 THO1: 6 TPOX: 8,9 vWA: 16
Name of Depositor：	C Helson
Deposited As：	Homo sapiens
Year of Origin：	1979
References：	Potluri VR, et al. Primitive neuroectodermal tumor cell lines: chromosomal analysis of five cases. Cancer Genet. Cytogenet. 24: 75-86, 1987. PubMed: 3024811 Schneider BS, et al. Expression of the cholecystokinin gene by cultured human primitive neuroepithelioma cell lines. J. Clin. Endocrinol. Metab. 69: 411-419, 1989. PubMed: 2753982 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.