SK-MEL-31

规格:

货期:

编号:TS210900

品牌:Testobio

产品名称：	SK-MEL-31
商品货号：	TS210900
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	epithelial cell
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Malignant melanoma
Gender：	female
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 82; range = 74 to 84. This is a hypotetra-/hypertriploid human cell line. The modal chromosome number is 82 occurring at 16% with polyploidy at 10.3%. The markers del(1)(q23), t(1q10p), del(7)(p13), t(7p18q), i(21q) and 5 others are common to all cells. They are all paired in most cells. N1 and N18 are absent, N11 is single-copied, and N6, N7, N8 and N16 are generally 4-copied (many cells also had 5 copies each for N7 and N8). The X is paired.
Derivation：	This is one of a very extensive series of melanoma lines (see also ATCC HTB-70, ATCC HTB-71 and ATCC HTB-72) isolated by T. Takahashi and associates.
Clinical Data：	female
Antigen Expression：	Antigen expression: HLA A3,A29, Bw35,B40, Cw3,Cw4, DRw4
Tumorigenic：	Yes
Effects：	Yes, in nude mice; produces malignant melanoma
Complete Growth Medium：	Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 85%; fetal bovine serum, 15%
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 13 D5S818: 11,13 D7S820: 10 THO1: 6,9 TPOX: 8,11 vWA: 16,18
Isoenzymes：	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1 PGM3, 1
Name of Depositor：	T Takahashi
Deposited As：	Homo sapiens
References：	Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619 Guldberg P, et al. Disruption of the MMAC1/PTEN gene by deletion or mutation is a frequent event in malignant melanoma. Cancer Res. 57: 3660-3663, 1997. PubMed: 9288767

首页 > 产品中心 > 微生物培养 > 菌株 > null > SK-MEL-31

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

SK-MEL-31

货号: TS210900
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	SK-MEL-31
商品货号：	TS210900
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	epithelial cell
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Malignant melanoma
Gender：	female
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 82; range = 74 to 84. This is a hypotetra-/hypertriploid human cell line. The modal chromosome number is 82 occurring at 16% with polyploidy at 10.3%. The markers del(1)(q23), t(1q10p), del(7)(p13), t(7p18q), i(21q) and 5 others are common to all cells. They are all paired in most cells. N1 and N18 are absent, N11 is single-copied, and N6, N7, N8 and N16 are generally 4-copied (many cells also had 5 copies each for N7 and N8). The X is paired.
Derivation：	This is one of a very extensive series of melanoma lines (see also ATCC HTB-70, ATCC HTB-71 and ATCC HTB-72) isolated by T. Takahashi and associates.
Clinical Data：	female
Antigen Expression：	Antigen expression: HLA A3,A29, Bw35,B40, Cw3,Cw4, DRw4
Tumorigenic：	Yes
Effects：	Yes, in nude mice; produces malignant melanoma
Complete Growth Medium：	Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 85%; fetal bovine serum, 15%
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 12 D16S539: 13 D5S818: 11,13 D7S820: 10 THO1: 6,9 TPOX: 8,11 vWA: 16,18
Isoenzymes：	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1 PGM3, 1
Name of Depositor：	T Takahashi
Deposited As：	Homo sapiens
References：	Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619 Guldberg P, et al. Disruption of the MMAC1/PTEN gene by deletion or mutation is a frequent event in malignant melanoma. Cancer Res. 57: 3660-3663, 1997. PubMed: 9288767