| 产品名称： | SK-N-DZ |
|---|---|
| 商品货号： | TS210904 |
| Organism： | Homo sapiens, human |
| Tissue： | brain; derived from metastatic site: bone marrow |
| Cell Type： | neuroblast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 2 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seen |
| Images： |  |
| Derivation： | SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma. |
| Clinical Data： | 2 years
Caucasian
female
|
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.
Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
|
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 8,11 D16S539: 9,11 D5S818: 12 D7S820: 12,13 THO1: 6,9 TPOX: 8 vWA: 16,18 |
| Name of Depositor： | C Helson |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1978 |
| References： | Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792 Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824 Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694 |
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| 产品名称： | SK-N-DZ |
|---|---|
| 商品货号： | TS210904 |
| Organism： | Homo sapiens, human |
| Tissue： | brain; derived from metastatic site: bone marrow |
| Cell Type： | neuroblast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 2 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seen |
| Images： | |
| Derivation： | SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma. |
| Clinical Data： | 2 years
Caucasian
female
|
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.
Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
|
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 8,11 D16S539: 9,11 D5S818: 12 D7S820: 12,13 THO1: 6,9 TPOX: 8 vWA: 16,18 |
| Name of Depositor： | C Helson |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1978 |
| References： | Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792 Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824 Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694 |
