| 产品名称： | SK-NEP-1 |
|---|---|
| 商品货号： | TS210905 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney; Derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | rounded |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 25 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | (P12) hypotriploid to hypertriploid (+A1, +A2, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, secondary constrictions and large subtelocentric markers |
| Clinical Data： | 25 years Caucasian female |
| Antigen Expression： | Antigen expression: Blood Type A; Rh+ |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms tumor with small cells consistent with Wilms tumor |
| Comments： | Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 105 cells/mL and maintain at between 105 and 106 cells/mL.
Medium Renewal: Every 2 to 4 days
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,10 THO1: 8,9.3 TPOX: 8,11 vWA: 15,19 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Orkin SH, et al. Development of homozygosity for chromosome 11p markers in Wilms tumour. Nature 309: 172-174, 1984. PubMed: 6325937 |
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| 产品名称： | SK-NEP-1 |
|---|---|
| 商品货号： | TS210905 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney; Derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | rounded |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 25 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | (P12) hypotriploid to hypertriploid (+A1, +A2, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, secondary constrictions and large subtelocentric markers |
| Clinical Data： | 25 years Caucasian female |
| Antigen Expression： | Antigen expression: Blood Type A; Rh+ |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms tumor with small cells consistent with Wilms tumor |
| Comments： | Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 105 cells/mL and maintain at between 105 and 106 cells/mL.
Medium Renewal: Every 2 to 4 days
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,10 THO1: 8,9.3 TPOX: 8,11 vWA: 15,19 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | G Trempe, LJ Old |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Orkin SH, et al. Development of homozygosity for chromosome 11p markers in Wilms tumour. Nature 309: 172-174, 1984. PubMed: 6325937 |
