SK-N-MC

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货期:

编号:TS210906

品牌:Testobio

产品名称：	SK-N-MC
商品货号：	TS210906
Organism：	Homo sapiens, human
Tissue：	brain; derived from metastatic site: supra-orbital area
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	neuroepithelioma
Age：	14 years
Gender：	female
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range and a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many (N1, N2, N4, N15, N16, N17, N21, and N22) are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. Numerous marker chromosomes are present including: 1p+, der(3)t(2;3)(q24;q27), del(4)(p12), 11q+, del(2)(q23), ampl.(17)(p12), ampl.(16)(q13), del(15)(q13q22), 21p+, iso(3q), del(22)(q11q13). Marker chromosomes M2 and M3 appear to us to be identical to two markers (M4 and M3, respectively) described by R.C. Seeger, et al.for this cell line. RefSeeger RC, et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461
Derivation：	This is one of two cell lines (see ATCC HTB-11) of neurogenic origin derived by J.L. Biedler. SK-N-MC was isolated in September of l971.
Clinical Data：	14 years Caucasian female
Antigen Expression：	Antigen expression: Blood Type O; Rh+
Genes Expressed：	Blood Type O; Rh+
Tumorigenic：	Yes
Effects：	Yes, in hamster cheek Yes, in nude mice
Comments：	This cell line was found to have moderate dopamine - beta - hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 12 D5S818: 11 D7S820: 8 THO1: 9.3 TPOX: 9,11 vWA: 17,18
Isoenzymes：	AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1-2
Name of Depositor：	G Trempe, LJ Old
Deposited As：	Homo sapiens
References：	Spengler BA, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells established in vitro. In Vitro 8: 410, 1973. Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Seeger RC, et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 Gromeier M, et al. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc. Natl. Acad. Sci. USA 93: 2370-2375, 1996. PubMed: 8637880 Seeger RC, et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461

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SK-N-MC

货号: TS210906
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产品名称：	SK-N-MC
商品货号：	TS210906
Organism：	Homo sapiens, human
Tissue：	brain; derived from metastatic site: supra-orbital area
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	neuroepithelioma
Age：	14 years
Gender：	female
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range and a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many (N1, N2, N4, N15, N16, N17, N21, and N22) are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. Numerous marker chromosomes are present including: 1p+, der(3)t(2;3)(q24;q27), del(4)(p12), 11q+, del(2)(q23), ampl.(17)(p12), ampl.(16)(q13), del(15)(q13q22), 21p+, iso(3q), del(22)(q11q13). Marker chromosomes M2 and M3 appear to us to be identical to two markers (M4 and M3, respectively) described by R.C. Seeger, et al.for this cell line. RefSeeger RC, et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461
Derivation：	This is one of two cell lines (see ATCC HTB-11) of neurogenic origin derived by J.L. Biedler. SK-N-MC was isolated in September of l971.
Clinical Data：	14 years Caucasian female
Antigen Expression：	Antigen expression: Blood Type O; Rh+
Genes Expressed：	Blood Type O; Rh+
Tumorigenic：	Yes
Effects：	Yes, in hamster cheek Yes, in nude mice
Comments：	This cell line was found to have moderate dopamine - beta - hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 12 D5S818: 11 D7S820: 8 THO1: 9.3 TPOX: 9,11 vWA: 17,18
Isoenzymes：	AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1-2
Name of Depositor：	G Trempe, LJ Old
Deposited As：	Homo sapiens
References：	Spengler BA, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells established in vitro. In Vitro 8: 410, 1973. Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Seeger RC, et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 Gromeier M, et al. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc. Natl. Acad. Sci. USA 93: 2370-2375, 1996. PubMed: 8637880 Seeger RC, et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461