SH-SY5Y

规格:

货期:

编号:TS210924

品牌:Testobio

产品名称：	SH-SY5Y
商品货号：	TS210924
Organism：	Homo sapiens, human
Tissue：	bone marrow
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	mixed, adherent and suspension
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	neuroblastoma
Age：	4 years
Gender：	female
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q RefRoss RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586
Images：
Derivation：	SH-SY5Y is a thrice cloned (SK-N-SH -> SH-SY -> SH-SY5 -> SH-SY5Y) subline of the neuroblastoma cell line SK-N-SH (see ATCC HTB-11) which was established in 1970 from a metastatic bone tumor.
Clinical Data：	4 years female
Antigen Expression：	Blood Type A; Rh+
Comments：	SH-SY5Y cells have a reported saturation density greater than 1 X 10⁶ cells/cm². They are reported to exhibit moderate levels of dopamine beta hydroxylase activity. RefBiedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704xa0
Complete Growth Medium：	The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	These cells grow as a mixture of floating and adherent cells. The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with fresh 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 mL of trypsin solution, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.xa0For slightly adherent cells that you don’t want to stick, usexa0Corning^® T-75 Ultra Low Attachment Flasks (catalog # 3814) to subculture this product. Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:50 is recommended Medium Renewal: Every 4 to 7 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 8,13 D5S818: 12 D7S820: 7,10 THO1: 7,10 TPOX: 8,11 vWA: 14,18
Population Doubling Time：	48 hrs
Name of Depositor：	JL Biedler
Deposited As：	Homo sapiens
Year of Origin：	1970
References：	Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586 Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704 Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586

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SH-SY5Y

货号: TS210924
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产品名称：	SH-SY5Y
商品货号：	TS210924
Organism：	Homo sapiens, human
Tissue：	bone marrow
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	mixed, adherent and suspension
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	neuroblastoma
Age：	4 years
Gender：	female
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q RefRoss RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586
Images：
Derivation：	SH-SY5Y is a thrice cloned (SK-N-SH -> SH-SY -> SH-SY5 -> SH-SY5Y) subline of the neuroblastoma cell line SK-N-SH (see ATCC HTB-11) which was established in 1970 from a metastatic bone tumor.
Clinical Data：	4 years female
Antigen Expression：	Blood Type A; Rh+
Comments：	SH-SY5Y cells have a reported saturation density greater than 1 X 10⁶ cells/cm². They are reported to exhibit moderate levels of dopamine beta hydroxylase activity. RefBiedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704xa0
Complete Growth Medium：	The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	These cells grow as a mixture of floating and adherent cells. The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with fresh 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 mL of trypsin solution, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.xa0For slightly adherent cells that you don’t want to stick, usexa0Corning^® T-75 Ultra Low Attachment Flasks (catalog # 3814) to subculture this product. Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:50 is recommended Medium Renewal: Every 4 to 7 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 8,13 D5S818: 12 D7S820: 7,10 THO1: 7,10 TPOX: 8,11 vWA: 14,18
Population Doubling Time：	48 hrs
Name of Depositor：	JL Biedler
Deposited As：	Homo sapiens
Year of Origin：	1970
References：	Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586 Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704 Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. PubMed: 6137586