SCC-25

规格:

货期:

编号:TS210942

品牌:Testobio

产品名称：	SCC-25
商品货号：	TS210942
Organism：	Homo sapiens, human
Tissue：	tongue
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	squamous cell carcinoma
Age：	70 years
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Images：
Clinical Data：	male 70 years
Genes Expressed：	epidermal keratins; low levels of involucrin
Cellular Products：	epidermal keratins; low levels of involucrin
Tumorigenic：	Yes
Effects：	Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10⁷ cells.
Comments：	SCC-25 cells are capable of only limited proliferation in semi-solid medium.
Complete Growth Medium：	A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate and supplemented with 400 ng/ml hydrocortisone and 10% fetal bovine serum
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Note: Cells are disaggregated to single cells when the culture is preconfluent. They do not require a feeder layer.
Cryopreservation：	Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: Air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 13 D16S539: 11,12 D5S818: 12 D7S820: 12 THO1: 8 TPOX: 8,12 vWA: 17,19
Name of Depositor：	JG Rheinwald
Deposited As：	Homo sapiens
References：	Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336

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SCC-25

货号: TS210942
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产品名称：	SCC-25
商品货号：	TS210942
Organism：	Homo sapiens, human
Tissue：	tongue
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	squamous cell carcinoma
Age：	70 years
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Images：
Clinical Data：	male 70 years
Genes Expressed：	epidermal keratins; low levels of involucrin
Cellular Products：	epidermal keratins; low levels of involucrin
Tumorigenic：	Yes
Effects：	Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10⁷ cells.
Comments：	SCC-25 cells are capable of only limited proliferation in semi-solid medium.
Complete Growth Medium：	A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate and supplemented with 400 ng/ml hydrocortisone and 10% fetal bovine serum
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Note: Cells are disaggregated to single cells when the culture is preconfluent. They do not require a feeder layer.
Cryopreservation：	Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: Air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 13 D16S539: 11,12 D5S818: 12 D7S820: 12 THO1: 8 TPOX: 8,12 vWA: 17,19
Name of Depositor：	JG Rheinwald
Deposited As：	Homo sapiens
References：	Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336