SBAC

规格:

货期:

编号:TS210957

品牌:Testobio

产品名称：	SBAC
商品货号：	TS210957
Organism：	Bos taurus, cow
Tissue：	adrenal gland, cortex; zona fasciculata; zona reticularis
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 Cells contain Bovine Viral Diarrhea Virus (BVDV) Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	adult
Applications：	The cells are responsive to ACTH and other steroidogenic agents. SBAC is a normal mammalian endocrine cell type that proliferates in culture, with or without serum, and maintains regulation of its differentiated functions. Fibroblast growth factor (FGF) stimulates cell division.
Storage Conditions：	liquid nitrogen vapor phase
Receptor Expression：	adrenocorticotropic hormone (ACTH)
Comments：	The cells are responsive to ACTH and other steroidogenic agents. SBAC is a normal mammalian endocrine cell type that proliferates in culture, with or without serum, and maintains regulation of its differentiated functions. Fibroblast growth factor (FGF) stimulates cell division. The cells have a finite life span in culture (60 to 70 population doublings). Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium：	Hams F12 medium with FGF (50 ng/ml of crude FGF - OR - 500 pg/ml of pure FGF), 90%; fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	MH Simonian
Deposited As：	Bos taurus
References：	Hornsby PJ, Gill GN. Hormonal control of adrenocortical cell proliferation. Desensitization to ACTH and interaction between ACTH and fibroblast growth factor in bovine adrenocortical cell cultures. J. Clin. Invest. 60: 342-352, 1977. PubMed: 194925 Simonian MH, et al. Clonal growth and culture life span of bovine adrenocortical cells in a serum-free medium. In Vitro Cell. Dev. Biol. 23: 247-252, 1987. PubMed: 3032889 Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Simonian MH, et al. Growth and function of cultured bovine adrenocortical cells in a serum-free defined medium. Endocrinology 111: 919-927, 1982. PubMed: 6213403 Gospodarowicz D, et al. Control of bovine adrenal cortical cell proliferation by fibroblast growth factor. Lack of effect of epidermal growth factor. Endocrinology 100: 1080-1089, 1977. PubMed: 189990 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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SBAC

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产品名称：	SBAC
商品货号：	TS210957
Organism：	Bos taurus, cow
Tissue：	adrenal gland, cortex; zona fasciculata; zona reticularis
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 Cells contain Bovine Viral Diarrhea Virus (BVDV) Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	adult
Applications：	The cells are responsive to ACTH and other steroidogenic agents. SBAC is a normal mammalian endocrine cell type that proliferates in culture, with or without serum, and maintains regulation of its differentiated functions. Fibroblast growth factor (FGF) stimulates cell division.
Storage Conditions：	liquid nitrogen vapor phase
Receptor Expression：	adrenocorticotropic hormone (ACTH)
Comments：	The cells are responsive to ACTH and other steroidogenic agents. SBAC is a normal mammalian endocrine cell type that proliferates in culture, with or without serum, and maintains regulation of its differentiated functions. Fibroblast growth factor (FGF) stimulates cell division. The cells have a finite life span in culture (60 to 70 population doublings). Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium：	Hams F12 medium with FGF (50 ng/ml of crude FGF - OR - 500 pg/ml of pure FGF), 90%; fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	MH Simonian
Deposited As：	Bos taurus
References：	Hornsby PJ, Gill GN. Hormonal control of adrenocortical cell proliferation. Desensitization to ACTH and interaction between ACTH and fibroblast growth factor in bovine adrenocortical cell cultures. J. Clin. Invest. 60: 342-352, 1977. PubMed: 194925 Simonian MH, et al. Clonal growth and culture life span of bovine adrenocortical cells in a serum-free medium. In Vitro Cell. Dev. Biol. 23: 247-252, 1987. PubMed: 3032889 Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Simonian MH, et al. Growth and function of cultured bovine adrenocortical cells in a serum-free defined medium. Endocrinology 111: 919-927, 1982. PubMed: 6213403 Gospodarowicz D, et al. Control of bovine adrenal cortical cell proliferation by fibroblast growth factor. Lack of effect of epidermal growth factor. Endocrinology 100: 1080-1089, 1977. PubMed: 189990 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.