| 产品名称： | RPMI 1846 |
|---|---|
| 商品货号： | TS210988 |
| Organism： | Mesocricetus auratus, hamster, Syrian golden |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | melanotic melanoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 67; range = 61 to 69 |
| Genes Expressed： | melanin |
| Cellular Products： | melanin |
| Tumorigenic： | Yes |
| Effects： | Yes, in newborn hamsters |
| Virus Susceptibility： | Vesicular stomatitis virus
Human poliovirus 1 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1: 2 to 1: 5 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.xa0xa0 |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2) |
| Name of Depositor： | GE Moore |
| Deposited As： | Mesocricetus auratus |
| References： | Moore GE, et al. Continuous culture of a melanotic cell line from the golden hamster. Science 137: 986-987, 1962. PubMed: 14475649 Mount D, et al. Culture of malignant tumors of the Syrian hamster. J. Natl. Cancer Inst. 31: 1217-1237, 1963. PubMed: 14071829 Hsu TC, Kellogg DS Jr.. Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens. J. Natl. Cancer Inst. 25: 221-235, 1960. PubMed: 14403619 Fortner JG, et al. Transplantable tumors of the Syrian (golden) hamster. I. Tumors of the alimentary tract, endocrine glands and melanomas. Cancer Res. 21: 161-198, 1961. PubMed: 13700922 |
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| 产品名称： | RPMI 1846 |
|---|---|
| 商品货号： | TS210988 |
| Organism： | Mesocricetus auratus, hamster, Syrian golden |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | melanotic melanoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 67; range = 61 to 69 |
| Genes Expressed： | melanin |
| Cellular Products： | melanin |
| Tumorigenic： | Yes |
| Effects： | Yes, in newborn hamsters |
| Virus Susceptibility： | Vesicular stomatitis virus
Human poliovirus 1 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1: 2 to 1: 5 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.xa0xa0 |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2) |
| Name of Depositor： | GE Moore |
| Deposited As： | Mesocricetus auratus |
| References： | Moore GE, et al. Continuous culture of a melanotic cell line from the golden hamster. Science 137: 986-987, 1962. PubMed: 14475649 Mount D, et al. Culture of malignant tumors of the Syrian hamster. J. Natl. Cancer Inst. 31: 1217-1237, 1963. PubMed: 14071829 Hsu TC, Kellogg DS Jr.. Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens. J. Natl. Cancer Inst. 25: 221-235, 1960. PubMed: 14403619 Fortner JG, et al. Transplantable tumors of the Syrian (golden) hamster. I. Tumors of the alimentary tract, endocrine glands and melanomas. Cancer Res. 21: 161-198, 1961. PubMed: 13700922 |
