| 产品名称： | RPMI 2650 |
|---|---|
| 商品货号： | TS210989 |
| Organism： | Homo sapiens, human |
| Tissue： | nasal septum; derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 52 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Genes Expressed： | mucoid; keratin |
| Cellular Products： | mucoid; keratin |
| Virus Susceptibility： | Human poliovirus 1
Herpes simplex virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) |
| Comments： | The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
NOTE: Cells attach in clusters. Cells will pile and the culture does not get 100% confluent. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| STR Profile： | Amelogenin: X,Y CSF1PO: 9,11 D13S317: 11,12 D16S539: 11,12 D5S818: 12,13 D7S820: 8,11 THO1: 6,8 TPOX: 8 vWA: 16,18 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Moore GE, Sandberg AA. Studies of a human tumor cell line with a diploid karyotype. Cancer 17: 170-175, 1964. PubMed: 14123677 Moorhead PS. Human tumor cell line with a quasi-diploid karyotype (RPMI 2650). Exp. Cell Res. 39: 190-196, 1965. PubMed: 5831238 |
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| 产品名称： | RPMI 2650 |
|---|---|
| 商品货号： | TS210989 |
| Organism： | Homo sapiens, human |
| Tissue： | nasal septum; derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 52 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Genes Expressed： | mucoid; keratin |
| Cellular Products： | mucoid; keratin |
| Virus Susceptibility： | Human poliovirus 1
Herpes simplex virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) |
| Comments： | The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
NOTE: Cells attach in clusters. Cells will pile and the culture does not get 100% confluent. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| STR Profile： | Amelogenin: X,Y CSF1PO: 9,11 D13S317: 11,12 D16S539: 11,12 D5S818: 12,13 D7S820: 8,11 THO1: 6,8 TPOX: 8 vWA: 16,18 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | GE Moore |
| Deposited As： | Homo sapiens |
| References： | Moore GE, Sandberg AA. Studies of a human tumor cell line with a diploid karyotype. Cancer 17: 170-175, 1964. PubMed: 14123677 Moorhead PS. Human tumor cell line with a quasi-diploid karyotype (RPMI 2650). Exp. Cell Res. 39: 190-196, 1965. PubMed: 5831238 |
