RIN-m5F

规格:

货期:

编号:TS211013

品牌:Testobio

产品名称：	RIN-m5F
商品货号：	TS211013
Organism：	Rattus norvegicus, rat
Tissue：	pancreas/islet of Langerhans
Cell Type：	beta cell
Product Format：	frozen
Morphology：	epithelial
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	insulinoma
Gender：	male
Strain：	NEDH
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images：
Derivation：	The RIN-m5F cell line is a clone derived from the RIN-m rat islet cell line.
Clinical Data：	male
Genes Expressed：	insulin; L-dopa-decarboxylase
Comments：	The cells produce and secrete insulin, and produce L-dopa-decarboxylase (a marker for cells having"amine precursor uptake and decarboxylation or APUD, activity). Unlike the parental line they do not produce somatostatin.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Cells will take about 3 days to attach fully. Cells attach as small islands which begin to flatten and spread into patchy epithelial morphology by day 3 or 4. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Two to three times weekly. Do not fluid change for 3 to 4 days after plating. Cells are loose. Just add medium.
Cryopreservation：	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	Miles, Inc.
Deposited As：	Rattus sp.
References：	Bhathena SJ, et al. Insulin, glucagon, and somatostatin secretion by cultured rat islet cell tumor and its clones. Proc. Soc. Exp. Biol. Med. 175: 35-38, 1984. PubMed: 6141566 Chick WL, et al. A transplantable insulinoma in the rat. Proc. Natl. Acad. Sci. USA 74: 628-632, 1977. PubMed: 191819 Bhathena SJ, et al. Insulin, glucagon, and somatostatin receptors on cultured cells and clones from rat islet cell tumor. Diabetes 31: 521-531, 1982. PubMed: 6295859 Gazdar AF, et al. Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. Proc. Natl. Acad. Sci. USA 77: 3519-3523, 1980. PubMed: 6106192 Oie HK, et al. Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor. Endocrinology 112: 1070-1075, 1983. PubMed: 6129963

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RIN-m5F

货号: TS211013
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产品名称：	RIN-m5F
商品货号：	TS211013
Organism：	Rattus norvegicus, rat
Tissue：	pancreas/islet of Langerhans
Cell Type：	beta cell
Product Format：	frozen
Morphology：	epithelial
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	insulinoma
Gender：	male
Strain：	NEDH
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images：
Derivation：	The RIN-m5F cell line is a clone derived from the RIN-m rat islet cell line.
Clinical Data：	male
Genes Expressed：	insulin; L-dopa-decarboxylase
Comments：	The cells produce and secrete insulin, and produce L-dopa-decarboxylase (a marker for cells having"amine precursor uptake and decarboxylation or APUD, activity). Unlike the parental line they do not produce somatostatin.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Cells will take about 3 days to attach fully. Cells attach as small islands which begin to flatten and spread into patchy epithelial morphology by day 3 or 4. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Two to three times weekly. Do not fluid change for 3 to 4 days after plating. Cells are loose. Just add medium.
Cryopreservation：	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	Miles, Inc.
Deposited As：	Rattus sp.
References：	Bhathena SJ, et al. Insulin, glucagon, and somatostatin secretion by cultured rat islet cell tumor and its clones. Proc. Soc. Exp. Biol. Med. 175: 35-38, 1984. PubMed: 6141566 Chick WL, et al. A transplantable insulinoma in the rat. Proc. Natl. Acad. Sci. USA 74: 628-632, 1977. PubMed: 191819 Bhathena SJ, et al. Insulin, glucagon, and somatostatin receptors on cultured cells and clones from rat islet cell tumor. Diabetes 31: 521-531, 1982. PubMed: 6295859 Gazdar AF, et al. Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. Proc. Natl. Acad. Sci. USA 77: 3519-3523, 1980. PubMed: 6106192 Oie HK, et al. Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor. Endocrinology 112: 1070-1075, 1983. PubMed: 6129963