R2C

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货期:

编号:TS211053

品牌:Testobio

产品名称：	R2C
商品货号：	TS211053
Organism：	Rattus norvegicus, rat
Tissue：	testis
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Leydig cell tumor
Age：	2 months
Gender：	male
Strain：	WFu
Applications：	transfection host
Storage Conditions：	liquid nitrogen vapor phase
Images：
Genes Expressed：	steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Cellular Products：	steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Virus Resistance：	poliovirus 2
Comments：	Stimulation by other hormones or cAMP is not required for hormone secretion. Reverse Transcript positive
Complete Growth Medium：	The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5% horse serum to a final concentration of 15%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution diluted 1:10 in PBS to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of the diluted Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Add fresh medium at least twice per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	G Sato
Deposited As：	Rattus sp.
References：	Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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R2C

货号: TS211053
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产品名称：	R2C
商品货号：	TS211053
Organism：	Rattus norvegicus, rat
Tissue：	testis
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Leydig cell tumor
Age：	2 months
Gender：	male
Strain：	WFu
Applications：	transfection host
Storage Conditions：	liquid nitrogen vapor phase
Images：
Genes Expressed：	steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Cellular Products：	steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Virus Resistance：	poliovirus 2
Comments：	Stimulation by other hormones or cAMP is not required for hormone secretion. Reverse Transcript positive
Complete Growth Medium：	The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5% horse serum to a final concentration of 15%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution diluted 1:10 in PBS to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of the diluted Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Add fresh medium at least twice per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	G Sato
Deposited As：	Rattus sp.
References：	Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.