PK(15) [PK15, PK-15]

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货期:

编号:TS211100

品牌:Testobio

产品名称：	PK(15) PK15, PK-15
商品货号：	TS211100
Organism：	Sus scrofa, pig
Tissue：	kidney
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Cellular Products：	plasminogen activator; keratin
Virus Susceptibility：	Classical swine fever virus , Classical swine fever virus African swine fever virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) Vaccinia virus Human adenovirus 4 Human adenovirus 5 Human Coxsackievirus B 2 Human Coxsackievirus B3 Human Coxsackievirus B4 Human Coxsackievirus B 5 Human Coxsackievirus B 6
Virus Resistance：	poliovirus 2
Comments：	The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources including the ATCC. The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that TS211100 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. The cell line harbors an endogenous C-type retrovirus. The cells are positive for porcine circovirus (PCV) antigens. The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer twice with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels.xa0 Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	culture medium 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C
Name of Depositor：	Cutter Laboratories, Inc.
Deposited As：	Sus scrofa
References：	Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (TS211100) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830 Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860 Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256 Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097 Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060 Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403 James A House, personal communication
Cross References：	Nucleotide (GenBank) : AJ133817 Porcine endogenous retrovirus type C proviral gag, pol and env genesand LTR (class A, clone 42). Nucleotide (GenBank) : AJ133816 Porcine endogenous retrovirus type C proviral gag, pol and env genes and LTR (class B, clone 33).

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PK(15) [PK15, PK-15]

货号: TS211100
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产品名称：	PK(15) PK15, PK-15
商品货号：	TS211100
Organism：	Sus scrofa, pig
Tissue：	kidney
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Cellular Products：	plasminogen activator; keratin
Virus Susceptibility：	Classical swine fever virus , Classical swine fever virus African swine fever virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) Vaccinia virus Human adenovirus 4 Human adenovirus 5 Human Coxsackievirus B 2 Human Coxsackievirus B3 Human Coxsackievirus B4 Human Coxsackievirus B 5 Human Coxsackievirus B 6
Virus Resistance：	poliovirus 2
Comments：	The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources including the ATCC. The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that TS211100 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. The cell line harbors an endogenous C-type retrovirus. The cells are positive for porcine circovirus (PCV) antigens. The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer twice with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels.xa0 Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	culture medium 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C
Name of Depositor：	Cutter Laboratories, Inc.
Deposited As：	Sus scrofa
References：	Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (TS211100) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830 Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860 Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256 Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097 Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060 Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403 James A House, personal communication
Cross References：	Nucleotide (GenBank) : AJ133817 Porcine endogenous retrovirus type C proviral gag, pol and env genesand LTR (class A, clone 42). Nucleotide (GenBank) : AJ133816 Porcine endogenous retrovirus type C proviral gag, pol and env genes and LTR (class B, clone 33).