| 产品名称： | pgsE-606 |
|---|---|
| 商品货号： | TS211112 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | PgsE-606 is a Chinese hamster ovary cell mutant partially deficient in heparin sulfate N sulfotransferase. The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate), and isolated by replica plating 35Ssulfate colony autoradiography. PgsE-606 produce an under sulfated form of heparin sulfate. |
| Derivation： | The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate), and isolated by replica plating 35Ssulfate colony autoradiography. |
| Clinical Data： | female |
| Comments： | PgsE-606 is a Chinese hamster ovary cell mutant partially deficient in heparin sulfate N sulfotransferase. The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate), and isolated by replica plating 35Ssulfate colony autoradiography. PgsE-606 produce an under sulfated form of heparin sulfate. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | JD Esko |
| Deposited As： | Cricetulus griseus |
| References： | Bame KJ, Esko JD. Undersulfated heparan sulfate in a Chinese hamster ovary cell mutant defective in heparan sulfate N-sulfotransferase. J. Biol. Chem. 264: 8059-8065, 1989. PubMed: 2524478 Bame KJ, et al. Biosynthesis of heparan sulfate. Coordination of polymer-modification reactions in a Chinese hamster ovary cell mutant defective in N-sulfotransferase. J. Biol. Chem. 266: 10287-10293, 1991. PubMed: 2037581 Bame KJ, et al. Coupling of N-deacetylation and N-sulfation in a Chinese hamster ovary cell mutant defective in heparan sulfate N-sulfotransferase. J. Biol. Chem. 266: 12461-12468, 1991. PubMed: 2061321 |
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| 产品名称： | pgsE-606 |
|---|---|
| 商品货号： | TS211112 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | PgsE-606 is a Chinese hamster ovary cell mutant partially deficient in heparin sulfate N sulfotransferase. The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate), and isolated by replica plating 35Ssulfate colony autoradiography. PgsE-606 produce an under sulfated form of heparin sulfate. |
| Derivation： | The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate), and isolated by replica plating 35Ssulfate colony autoradiography. |
| Clinical Data： | female |
| Comments： | PgsE-606 is a Chinese hamster ovary cell mutant partially deficient in heparin sulfate N sulfotransferase. The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate), and isolated by replica plating 35Ssulfate colony autoradiography. PgsE-606 produce an under sulfated form of heparin sulfate. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | JD Esko |
| Deposited As： | Cricetulus griseus |
| References： | Bame KJ, Esko JD. Undersulfated heparan sulfate in a Chinese hamster ovary cell mutant defective in heparan sulfate N-sulfotransferase. J. Biol. Chem. 264: 8059-8065, 1989. PubMed: 2524478 Bame KJ, et al. Biosynthesis of heparan sulfate. Coordination of polymer-modification reactions in a Chinese hamster ovary cell mutant defective in N-sulfotransferase. J. Biol. Chem. 266: 10287-10293, 1991. PubMed: 2037581 Bame KJ, et al. Coupling of N-deacetylation and N-sulfation in a Chinese hamster ovary cell mutant defective in heparan sulfate N-sulfotransferase. J. Biol. Chem. 266: 12461-12468, 1991. PubMed: 2061321 |
