| 产品名称： | pgsD-677 |
|---|---|
| 商品货号： | TS211114 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | heparan sulfate deficient |
| Gender： | female |
| Derivation： | The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis. |
| Clinical Data： | female |
| Comments： | PgsD-677 is a Chinese hamster ovary cell mutant deficient in the polymerization of heparan sulfate.
PgsD-677 cells do not produce heparan sulfate. They do produce chondroitin sulfate. They lack both N-acetylglucosaminyltransferase and glucuronyltransferase activities that are required for synthesis of heparan sulfate.
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:8 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | JD Esko |
| Deposited As： | Cricetulus griseus |
| References： | Lidholt K, et al. A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis. Proc. Natl. Acad. Sci. USA 89: 2267-2271, 1992. PubMed: 1532254 Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658 |
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| 产品名称： | pgsD-677 |
|---|---|
| 商品货号： | TS211114 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | heparan sulfate deficient |
| Gender： | female |
| Derivation： | The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis. |
| Clinical Data： | female |
| Comments： | PgsD-677 is a Chinese hamster ovary cell mutant deficient in the polymerization of heparan sulfate.
PgsD-677 cells do not produce heparan sulfate. They do produce chondroitin sulfate. They lack both N-acetylglucosaminyltransferase and glucuronyltransferase activities that are required for synthesis of heparan sulfate.
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:8 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | JD Esko |
| Deposited As： | Cricetulus griseus |
| References： | Lidholt K, et al. A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis. Proc. Natl. Acad. Sci. USA 89: 2267-2271, 1992. PubMed: 1532254 Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658 |
