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NF-1
NF-1
规格:
货期:
编号:TS211219
品牌:Testobio
产品名称: NF-1
商品货号: TS211219
Organism: Mus musculus, mouse
Product Format: frozen
Morphology: epithelial
Culture Properties: mixed adherent-suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: nullipotent embryonal carcinoma; teratocarcinoma
Age: embryo; 7 days gestation
Gender: male
Strain: BALB/c
Applications:
This pluripotent stem cell line was established in 1979 by Fox et al.
Derivation:
This pluripotent stem cell line was established in 1979 by Fox et al.
Clinical Data:
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals.
When injected into male mice, fewer, and slower growing, tumors are formed.
male
Tumorigenic: Yes
Effects:
Yes, when injected intramuscularly into syngeneic mice
Comments:
This pluripotent stem cell line was established in 1979 by Fox et al.
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals.
When injected into male mice, fewer, and slower growing, tumors are formed.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
This is a mixed cell culture; cells grow both as a monolayer and as a suspension. Subcultures are prepared by scraping the attached cell into the medium containing the floaters. Aspirate and dispense into new culture flasks. Subculture approximately two times weekly. Cells will detach from surface of flask before confluency is reached.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: I Damjanov
Deposited As: Mus musculus
Year of Origin: 1979
References:

Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchows Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898

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NF-1

  • 货号: TS211219
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  • 品牌 : TESTOBIO
产品名称: NF-1
商品货号: TS211219
Organism: Mus musculus, mouse
Product Format: frozen
Morphology: epithelial
Culture Properties: mixed adherent-suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: nullipotent embryonal carcinoma; teratocarcinoma
Age: embryo; 7 days gestation
Gender: male
Strain: BALB/c
Applications:
This pluripotent stem cell line was established in 1979 by Fox et al.
Derivation:
This pluripotent stem cell line was established in 1979 by Fox et al.
Clinical Data:
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals.
When injected into male mice, fewer, and slower growing, tumors are formed.
male
Tumorigenic: Yes
Effects:
Yes, when injected intramuscularly into syngeneic mice
Comments:
This pluripotent stem cell line was established in 1979 by Fox et al.
When injected into the tail veins of female mice, the cells have a tendency to metastasize to the ovaries and adrenals.
When injected into male mice, fewer, and slower growing, tumors are formed.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
This is a mixed cell culture; cells grow both as a monolayer and as a suspension. Subcultures are prepared by scraping the attached cell into the medium containing the floaters. Aspirate and dispense into new culture flasks. Subculture approximately two times weekly. Cells will detach from surface of flask before confluency is reached.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: I Damjanov
Deposited As: Mus musculus
Year of Origin: 1979
References:

Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchows Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898

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