| 产品名称： | NCTC clone 3526 derivative of LLC-MK2 |
|---|---|
| 商品货号： | TS211236 |
| Organism： | Macaca mulatta, monkey, rhesus |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Applications： | The cells are positive for keratin by immunoperoxidase staining. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | The cells are positive for keratin by immunoperoxidase staining. keratin,The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products： | keratin |
| Comments： | Grows in chemically defined medium. The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | NCTC 135 medium, 100%
|
| Subculturing： | Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask. Aspirate and dispense into new flasks. The initial subculture interval after the cells are thawed may be somewhat extended (18 to 21 days). Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% Carbon dioxide (CO2) |
| Name of Depositor： | VJ Evans |
| Deposited As： | Macaca mulatta |
| References： | Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231 J. Natl. Cancer Inst. 29: 749, 1962. Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902 . . Am. J. Hyg. 70: 275, 1959. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | NCTC clone 3526 derivative of LLC-MK2 |
|---|---|
| 商品货号： | TS211236 |
| Organism： | Macaca mulatta, monkey, rhesus |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Applications： | The cells are positive for keratin by immunoperoxidase staining. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | The cells are positive for keratin by immunoperoxidase staining. keratin,The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products： | keratin |
| Comments： | Grows in chemically defined medium. The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | NCTC 135 medium, 100%
|
| Subculturing： | Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask. Aspirate and dispense into new flasks. The initial subculture interval after the cells are thawed may be somewhat extended (18 to 21 days). Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% Carbon dioxide (CO2) |
| Name of Depositor： | VJ Evans |
| Deposited As： | Macaca mulatta |
| References： | Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231 J. Natl. Cancer Inst. 29: 749, 1962. Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902 . . Am. J. Hyg. 70: 275, 1959. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
