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NCI-H838 [H838]
NCI-H838 [H838]
规格:
货期:
编号:TS211242
品牌:Testobio
产品名称: NCI-H838 H838
商品货号: TS211242
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: lymph node
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: 3B, adenocarcinoma; non-small cell lung cancer
Age: 59
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in October 1984
Clinical Data:
59
Caucasian
male
The patient was a smoker. 80 pack years
Comments:
The patient was a smoker. 80 pack years.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing:
xa0Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 mL by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C. Split ratio: 1:3 to 1:6.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile:
Amelogenin: X
CSF1PO: 10
D13S317: 8
D16S539: 9,12
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,11
vWA: 17
Name of Depositor: AF Gazdar, JD Minna
Year of Origin: 1984
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H838 [H838]

  • 货号: TS211242
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H838 H838
商品货号: TS211242
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: lymph node
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: 3B, adenocarcinoma; non-small cell lung cancer
Age: 59
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in October 1984
Clinical Data:
59
Caucasian
male
The patient was a smoker. 80 pack years
Comments:
The patient was a smoker. 80 pack years.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing:
xa0Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 mL by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C. Split ratio: 1:3 to 1:6.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile:
Amelogenin: X
CSF1PO: 10
D13S317: 8
D16S539: 9,12
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,11
vWA: 17
Name of Depositor: AF Gazdar, JD Minna
Year of Origin: 1984
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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