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NCI-H720 [H720]
NCI-H720 [H720]
规格:
货期:
编号:TS211249
品牌:Testobio
产品名称: NCI-H720 H720
商品货号: TS211249
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: atypical carcinoid
Gender: male
Storage Conditions: liquid nitrogen vapor phase
Karyotype: del(p14-p23), t(3p;4p)
Derivation: The line was established in April 1984.
Clinical Data:
The tissue donor was a non-smoker.

Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing: Cultures can be maintained by the addition of fresh medium.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation:
Culture medium, 95%; DMSO, 5.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1984
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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NCI-H720 [H720]

  • 货号: TS211249
  • 好评
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  • 品牌 : TESTOBIO
产品名称: NCI-H720 H720
商品货号: TS211249
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: atypical carcinoid
Gender: male
Storage Conditions: liquid nitrogen vapor phase
Karyotype: del(p14-p23), t(3p;4p)
Derivation: The line was established in April 1984.
Clinical Data:
The tissue donor was a non-smoker.

Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing: Cultures can be maintained by the addition of fresh medium.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation:
Culture medium, 95%; DMSO, 5.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1984
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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