| 产品名称： | NCI-H716 H716 |
|---|---|
| 商品货号： | TS211252 |
| Organism： | Homo sapiens, human |
| Tissue： | cecum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension, multicell aggregates and some adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | colorectal adenocarcinoma |
| Age： | 33 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 61; range = 55 to 64. This is a hypotriploid human cell line. The modal chromosome number is 61, occurring in 28% of cells. The rate of higher ploidies is 1.2%. Twelve marker chromosomes are common in these cells. These include t(7q,16q), t(5p,7p), t(4q,9q) and 9 others. A large number of DMs is found in every cell. The N21 is absent; and N9, N11, N13, N20, N22 and X are single-copied. No Y chromosome was detected in the Q-banded slide preparation. |
| Images： |  |
| Derivation： | This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil. |
| Clinical Data： | 33 years
Caucasian
male
|
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | The cells contain Dopa decarboxylase and, unlike other colorectal lines, contain cytoplasmic dense core granules characteristic of endocrine secretion. The cells do not express the TAG-72 or CA19-9 antigens nor do they produce carcinoembryonic antigen (CEA). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium Cultures can be established by centrifugation with subsequent resuspension at 4 to 8 x 105 viable cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 11,12
D5S818: 11
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1984 |
| References： | Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249 |
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| 产品名称： | NCI-H716 H716 |
|---|---|
| 商品货号： | TS211252 |
| Organism： | Homo sapiens, human |
| Tissue： | cecum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension, multicell aggregates and some adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | colorectal adenocarcinoma |
| Age： | 33 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 61; range = 55 to 64. This is a hypotriploid human cell line. The modal chromosome number is 61, occurring in 28% of cells. The rate of higher ploidies is 1.2%. Twelve marker chromosomes are common in these cells. These include t(7q,16q), t(5p,7p), t(4q,9q) and 9 others. A large number of DMs is found in every cell. The N21 is absent; and N9, N11, N13, N20, N22 and X are single-copied. No Y chromosome was detected in the Q-banded slide preparation. |
| Images： | |
| Derivation： | This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil. |
| Clinical Data： | 33 years
Caucasian
male
|
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | The cells contain Dopa decarboxylase and, unlike other colorectal lines, contain cytoplasmic dense core granules characteristic of endocrine secretion. The cells do not express the TAG-72 or CA19-9 antigens nor do they produce carcinoembryonic antigen (CEA). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium Cultures can be established by centrifugation with subsequent resuspension at 4 to 8 x 105 viable cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 11,12
D5S818: 11
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | AF Gazdar |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1984 |
| References： | Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249 |
