| 产品名称： | NCI-H358 H-358, H358 |
|---|---|
| 商品货号： | TS211269 |
| Organism： | Homo sapiens, human |
| Tissue： | lung/bronchiole; derived from metastatic site: alveolus |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | bronchioalveolar carcinoma; non-small cell lung cancer |
| Gender： | male |
| Applications： | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Images： |  |
| Derivation： | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. |
| Clinical Data： | male
|
| Genes Expressed： | lung surfactant associated protein A (SP-A),The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. |
| Cellular Products： | lung surfactant associated protein A (SP-A) |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells produce tumors in athymic nude mice |
| Comments： | Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. SP-B and SP-C RNA was not expressed. They have a reported colony forming efficiency of 0.83% in soft agarose. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 12,13 D5S818: 10,12 D7S820: 10,11 THO1: 6 TPOX: 8,9 vWA: 17 |
| Population Doubling Time： | 38 hrs |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1981 |
| References： | Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455 |
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| 产品名称： | NCI-H358 H-358, H358 |
|---|---|
| 商品货号： | TS211269 |
| Organism： | Homo sapiens, human |
| Tissue： | lung/bronchiole; derived from metastatic site: alveolus |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | bronchioalveolar carcinoma; non-small cell lung cancer |
| Gender： | male |
| Applications： | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Images： | |
| Derivation： | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. |
| Clinical Data： | male
|
| Genes Expressed： | lung surfactant associated protein A (SP-A),The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. |
| Cellular Products： | lung surfactant associated protein A (SP-A) |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells produce tumors in athymic nude mice |
| Comments： | Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. SP-B and SP-C RNA was not expressed. They have a reported colony forming efficiency of 0.83% in soft agarose. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 12,13 D5S818: 10,12 D7S820: 10,11 THO1: 6 TPOX: 8,9 vWA: 17 |
| Population Doubling Time： | 38 hrs |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1981 |
| References： | Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455 |
