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NCI-H28 [H28]
NCI-H28 [H28]
规格:
货期:
编号:TS211274
品牌:Testobio
产品名称: NCI-H28 H28
商品货号: TS211274
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: pleural effusion
Product Format: frozen
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage 4, mesothelioma
Age: 48 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Karyotype: t(3p;14q)
Derivation:
The line was established in September 1976.
Clinical Data:
48 years
Caucasian
male
The patient was a smoker.
Comments:
Complete Growth Medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing: Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,13
D16S539: 12,13
D5S818: 11
D7S820: 10,12
THO1: 7,9
TPOX: 11
vWA: 14,15
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: September, 1976
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H28 [H28]

  • 货号: TS211274
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H28 H28
商品货号: TS211274
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: pleural effusion
Product Format: frozen
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage 4, mesothelioma
Age: 48 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Karyotype: t(3p;14q)
Derivation:
The line was established in September 1976.
Clinical Data:
48 years
Caucasian
male
The patient was a smoker.
Comments:
Complete Growth Medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing: Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,13
D16S539: 12,13
D5S818: 11
D7S820: 10,12
THO1: 7,9
TPOX: 11
vWA: 14,15
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: September, 1976
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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