NCI-H2342 [H2342]

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货期:

编号:TS211282

品牌:Testobio

产品名称：	NCI-H2342 H2342
商品货号：	TS211282
Organism：	Homo sapiens, human
Tissue：	lung
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	stage 3A, adenocarcinoma; non-small cell lung cancer
Age：	55 years
Gender：	male
Ethnicity：	Caucasian
Derivation：	The line was established in April 1990 from lung adenocarcinoma.
Clinical Data：	55 years Caucasian male The tissue donor was a non-smoker.
Complete Growth Medium：	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:6.
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 13 D5S818: 12 D7S820: 10 THO1: 9,9.3 TPOX: 8,10 vWA: 17
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	1990
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H2342 [H2342]

货号: TS211282
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产品名称：	NCI-H2342 H2342
商品货号：	TS211282
Organism：	Homo sapiens, human
Tissue：	lung
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	stage 3A, adenocarcinoma; non-small cell lung cancer
Age：	55 years
Gender：	male
Ethnicity：	Caucasian
Derivation：	The line was established in April 1990 from lung adenocarcinoma.
Clinical Data：	55 years Caucasian male The tissue donor was a non-smoker.
Complete Growth Medium：	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:6.
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 13 D5S818: 12 D7S820: 10 THO1: 9,9.3 TPOX: 8,10 vWA: 17
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	1990
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.