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NCI-H220 [H220]
NCI-H220 [H220]
规格:
货期:
编号:TS211291
品牌:Testobio
产品名称: NCI-H220 H220
商品货号: TS211291
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Culture Properties: suspension, floating aggregates of round cells with some attached cells
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E,carcinoma; classic small cell lung cancer
Age: 51 years
Gender: male
Ethnicity: Black
Applications:
The line was established in February 1980.
Derivation:
The line was established in February 1980.
Clinical Data:
The tissue donor was a non-smoker.
male
Black
51 years
Comments:
The line was established in February 1980.
The tissue donor was a non-smoker.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 3 to 5 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
STR Profile: Amelogenin: X,Y
CSF1PO: 10
D13S317: 12
D16S539: 10
D5S818: 11
D7S820: 10, 11
TH01: 7,9.3
TPOX: 8,11
vWA: 16,18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1980
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H220 [H220]

  • 货号: TS211291
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H220 H220
商品货号: TS211291
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Culture Properties: suspension, floating aggregates of round cells with some attached cells
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E,carcinoma; classic small cell lung cancer
Age: 51 years
Gender: male
Ethnicity: Black
Applications:
The line was established in February 1980.
Derivation:
The line was established in February 1980.
Clinical Data:
The tissue donor was a non-smoker.
male
Black
51 years
Comments:
The line was established in February 1980.
The tissue donor was a non-smoker.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 3 to 5 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
STR Profile: Amelogenin: X,Y
CSF1PO: 10
D13S317: 12
D16S539: 10
D5S818: 11
D7S820: 10, 11
TH01: 7,9.3
TPOX: 8,11
vWA: 16,18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1980
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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