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NCI-H2195 [H2195]
NCI-H2195 [H2195]
规格:
货期:
编号:TS211292
品牌:Testobio
产品名称: NCI-H2195 H2195
商品货号: TS211292
Organism: Homo sapiens, human
Tissue: lung; derived from metatstatic site: bone marrow
Cell Type: lymphoblast
Product Format: frozen
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E, carcinoma; small cell lung cancer
Age: 67 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in June 1989 from bone marrow metastasis of a lung.
Clinical Data:
67 yearsxa0
Caucasian
male
Comments:
A lymphoblastoid line from the same patient is available as ATCC CRL-5956.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
  1. Remove medium, rinse with 0.25% (w/v) trypsin - 0.53 mM EDTA solution
  2. Add 1 to 2 mL additional trypsin solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach.
  3. Resuspend pellet in fresh medium and dispense into a culture flask.
Subcultivation Ratio: A ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice weekly
Cryopreservation:
Freeze medium: Culture medium, 90%; DMSO, 5%; FBS ,5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 11
D13S317: 11
D16S539: 10
D5S818: 12
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1989
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

100 pack years

The line was established from tissue taken from a patient with a 100 pack-year history of smoking.

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NCI-H2195 [H2195]

  • 货号: TS211292
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H2195 H2195
商品货号: TS211292
Organism: Homo sapiens, human
Tissue: lung; derived from metatstatic site: bone marrow
Cell Type: lymphoblast
Product Format: frozen
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E, carcinoma; small cell lung cancer
Age: 67 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in June 1989 from bone marrow metastasis of a lung.
Clinical Data:
67 yearsxa0
Caucasian
male
Comments:
A lymphoblastoid line from the same patient is available as ATCC CRL-5956.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
  1. Remove medium, rinse with 0.25% (w/v) trypsin - 0.53 mM EDTA solution
  2. Add 1 to 2 mL additional trypsin solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach.
  3. Resuspend pellet in fresh medium and dispense into a culture flask.
Subcultivation Ratio: A ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice weekly
Cryopreservation:
Freeze medium: Culture medium, 90%; DMSO, 5%; FBS ,5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 11
D13S317: 11
D16S539: 10
D5S818: 12
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1989
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

100 pack years

The line was established from tissue taken from a patient with a 100 pack-year history of smoking.

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