你好,请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
联系电话: call_new 0574-87917803
NCI-H2198 [H2198]
NCI-H2198 [H2198]
规格:
货期:
编号:TS211293
品牌:Testobio
产品名称: NCI-H2198 H2198
商品货号: TS211293
Organism: Homo sapiens, human
Tissue: lung; Derived from metastatic site: lymph node
Product Format: frozen
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E,carcinoma; small cell lung cancer
Age: 67 years
Gender: male
Ethnicity: Caucasian
Applications:
The line was established in June 1989.
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in June 1989.
Clinical Data:
The patient was a smoker.
male
Caucasian
67 years
Comments:
The line was established in June 1989.
The patient was a smoker.
100 pack years.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation:
Freeze medium: 50% FBS + 40% Growth Medium + 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1989
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

首页 > 产品中心 > 微生物培养 > 菌株 > null > NCI-H2198 [H2198]

NCI-H2198 [H2198]

  • 货号: TS211293
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H2198 H2198
商品货号: TS211293
Organism: Homo sapiens, human
Tissue: lung; Derived from metastatic site: lymph node
Product Format: frozen
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E,carcinoma; small cell lung cancer
Age: 67 years
Gender: male
Ethnicity: Caucasian
Applications:
The line was established in June 1989.
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in June 1989.
Clinical Data:
The patient was a smoker.
male
Caucasian
67 years
Comments:
The line was established in June 1989.
The patient was a smoker.
100 pack years.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation:
Freeze medium: 50% FBS + 40% Growth Medium + 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Temperature: 37.0°C
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1989
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

合作单位: