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NCI-H2106 [H2106]
NCI-H2106 [H2106]
规格:
货期:
编号:TS211298
品牌:Testobio
产品名称: NCI-H2106 H2106
商品货号: TS211298
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: lymph node
Product Format: frozen
Morphology: floating clusters
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage 4, non-small cell lung cancer
Age: 58 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in December 1988 from lymph node, a metastatic site.
The patient received prior chemotherapy and radiation therapy.
The tissue donor was a non-smoker.
Clinical Data:
58 years
male
Caucasian
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation:
Culture medium with an additional 5% fetal bovine serum, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions:
Temperature: 37°C
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1988
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

首页 > 产品中心 > 微生物培养 > 菌株 > null > NCI-H2106 [H2106]

NCI-H2106 [H2106]

  • 货号: TS211298
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H2106 H2106
商品货号: TS211298
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: lymph node
Product Format: frozen
Morphology: floating clusters
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage 4, non-small cell lung cancer
Age: 58 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in December 1988 from lymph node, a metastatic site.
The patient received prior chemotherapy and radiation therapy.
The tissue donor was a non-smoker.
Clinical Data:
58 years
male
Caucasian
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation:
Culture medium with an additional 5% fetal bovine serum, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions:
Temperature: 37°C
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1988
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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