NCI-H2122 [H2122]

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编号:TS211305

品牌:Testobio

产品名称：	NCI-H2122 H2122
商品货号：	TS211305
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Cell Type：	lymphoblast
Product Format：	frozen
Morphology：	it is normal for CRL-5985 to show a mixed morphology of rounded cells and epithelial cells. This cell line produces moderate to large, viable, loose, grape-like clusters that either loosely adhere to the flask or remain in suspension
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	stage 4, adenocarcinoma; non-small cell lung cancer
Age：	46 years
Gender：	female
Ethnicity：	Caucasian, White
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was established in January 1989 from pleural effusion metastasis of lung.
Clinical Data：	46 years Caucasian female The patient was a smoker 30 pack year
Comments：	A lymphoblastoid line from the same patient is available as ATCC CRL-5967.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 10 D16S539: 9,12 D5S818: 11,12 D7S820: 8,10 THO1: 7,9.3 TPOX: 9 vWA: 17
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	January, 1989
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H2122 [H2122]

货号: TS211305
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产品名称：	NCI-H2122 H2122
商品货号：	TS211305
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Cell Type：	lymphoblast
Product Format：	frozen
Morphology：	it is normal for CRL-5985 to show a mixed morphology of rounded cells and epithelial cells. This cell line produces moderate to large, viable, loose, grape-like clusters that either loosely adhere to the flask or remain in suspension
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	stage 4, adenocarcinoma; non-small cell lung cancer
Age：	46 years
Gender：	female
Ethnicity：	Caucasian, White
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was established in January 1989 from pleural effusion metastasis of lung.
Clinical Data：	46 years Caucasian female The patient was a smoker 30 pack year
Comments：	A lymphoblastoid line from the same patient is available as ATCC CRL-5967.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 10 D16S539: 9,12 D5S818: 11,12 D7S820: 8,10 THO1: 7,9.3 TPOX: 9 vWA: 17
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	January, 1989
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.