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NCI-H2135 [H2135]
NCI-H2135 [H2135]
规格:
货期:
编号:TS211306
品牌:Testobio
产品名称: NCI-H2135 H2135
商品货号: TS211306
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: non-small cell lung cancer
Applications:
The line was established in February 1989.
Derivation:
The line was established in February 1989.
Clinical Data:
The tissue donor was a non-smoker.
Comments:
The line was established in February 1989.
The tissue donor was a non-smoker.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 2 X 10 exp5 and 5 X 10 exp5 viable cells/ml.
Adherent cells may be harvested by scraping.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
STR Profile:
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 12,13
D7S820: 11,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1989
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H2135 [H2135]

  • 货号: TS211306
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询价
  • 品牌 : TESTOBIO
产品名称: NCI-H2135 H2135
商品货号: TS211306
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: non-small cell lung cancer
Applications:
The line was established in February 1989.
Derivation:
The line was established in February 1989.
Clinical Data:
The tissue donor was a non-smoker.
Comments:
The line was established in February 1989.
The tissue donor was a non-smoker.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 2 X 10 exp5 and 5 X 10 exp5 viable cells/ml.
Adherent cells may be harvested by scraping.
Cryopreservation:
Culture medium, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
STR Profile:
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 12,13
D7S820: 11,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1989
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

合作单位: