NCI-H2126 [H2126]

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货期:

编号:TS211307

品牌:Testobio

产品名称：	NCI-H2126 H2126
商品货号：	TS211307
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	adenocarcinoma; non-small cell lung cancer
Age：	65 years adult
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	Modal number = 79; range = 71 to 83 This is a hypertriploid human cell line with the modal chromosome number of 79, occurring in 20% of cells. The rate of cells with a higher ploidy was 5.5%. Karyotypes were very complex. There were over 22 marker chromosomes commonly present in most cells, many with complex structural rearrangements. Among these markers were: double copies for t(10qter--10q11.2::?::13C--13qter) and der(9)t(3;9) (p12;p22?), and one copy each for del (1) (p22) and i (iq). There were two normal X chromosomes per cell. Normal chromosomes Y, N1, N5, N14, and N15 were not found. Chromosomes N20 and N22 generally had four or more copies per cell.
Clinical Data：	65 years adult Caucasian male
HeLa Markers：	N
Tumorigenic：	Yes
Effects：	Yes, in nude mice (Tumors developed within one month in 4/5 nude mice inoculated subcutaneously with 10(7) cells.)
Comments：	An EBV-transformed lymphoblastoid cell line from the same patient is available as TS211307.1.
Complete Growth Medium：	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. xa0Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum whichxa0xa0 contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cellxa0xa0 layer isxa0 dispersed (usually within 5 to 15 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspiratexa0 cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culturexa0 vessels. An inoculum of 6 x 10³ to 8 x 10³ viable cells/cm² is recommended. Or use Subcultivation Ratio: 1:2 to 1:4 Incubate cultures at 37°C. Subculture when reaching a cell concentration betweenxa0 8 x 10⁴ and 1 x 10⁵ cells/cm². Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 11 D13S317: 12,14 D16S539: 12 D5S818: 11 D7S820: 8,9 THO1: 7,9.3 TPOX: 8 vWA: 17
Isoenzymes：	AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 2
Population Doubling Time：	about 41 hours
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens

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NCI-H2126 [H2126]

货号: TS211307
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产品名称：	NCI-H2126 H2126
商品货号：	TS211307
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	adenocarcinoma; non-small cell lung cancer
Age：	65 years adult
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	Modal number = 79; range = 71 to 83 This is a hypertriploid human cell line with the modal chromosome number of 79, occurring in 20% of cells. The rate of cells with a higher ploidy was 5.5%. Karyotypes were very complex. There were over 22 marker chromosomes commonly present in most cells, many with complex structural rearrangements. Among these markers were: double copies for t(10qter--10q11.2::?::13C--13qter) and der(9)t(3;9) (p12;p22?), and one copy each for del (1) (p22) and i (iq). There were two normal X chromosomes per cell. Normal chromosomes Y, N1, N5, N14, and N15 were not found. Chromosomes N20 and N22 generally had four or more copies per cell.
Clinical Data：	65 years adult Caucasian male
HeLa Markers：	N
Tumorigenic：	Yes
Effects：	Yes, in nude mice (Tumors developed within one month in 4/5 nude mice inoculated subcutaneously with 10(7) cells.)
Comments：	An EBV-transformed lymphoblastoid cell line from the same patient is available as TS211307.1.
Complete Growth Medium：	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. xa0Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum whichxa0xa0 contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cellxa0xa0 layer isxa0 dispersed (usually within 5 to 15 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspiratexa0 cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culturexa0 vessels. An inoculum of 6 x 10³ to 8 x 10³ viable cells/cm² is recommended. Or use Subcultivation Ratio: 1:2 to 1:4 Incubate cultures at 37°C. Subculture when reaching a cell concentration betweenxa0 8 x 10⁴ and 1 x 10⁵ cells/cm². Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 11 D13S317: 12,14 D16S539: 12 D5S818: 11 D7S820: 8,9 THO1: 7,9.3 TPOX: 8 vWA: 17
Isoenzymes：	AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 2
Population Doubling Time：	about 41 hours
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens