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NCI-H2023 [H2023]
NCI-H2023 [H2023]
规格:
货期:
编号:TS211312
品牌:Testobio
产品名称: NCI-H2023 H2023
商品货号: TS211312
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage 3A, adenocarcinoma; non-small cell lung cancer
Age: 26 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in September 1988 from lymph node, a metastatic site.
Clinical Data:
The tissue donor was a non-smoker.

Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Cryopreservation:
Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
STR Profile:
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 12
D7S820: 11
THO1: 7,9
TPOX: 8
vWA: 18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1988
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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NCI-H2023 [H2023]

  • 货号: TS211312
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H2023 H2023
商品货号: TS211312
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage 3A, adenocarcinoma; non-small cell lung cancer
Age: 26 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in September 1988 from lymph node, a metastatic site.
Clinical Data:
The tissue donor was a non-smoker.

Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Cryopreservation:
Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
STR Profile:
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 12
D7S820: 11
THO1: 7,9
TPOX: 8
vWA: 18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1988
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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