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NCI-H1882 [H1882]
NCI-H1882 [H1882]
规格:
货期:
编号:TS211318
品牌:Testobio
产品名称： NCI-H1882 H1882
商品货号： TS211318
Organism： Homo sapiens, human
Tissue： lung; Derived from metastatic site: bone marrow
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： stage E,carcinoma; small cell lung cancer
Age： 59 years
Gender： male
Ethnicity： Caucasian
Applications：
The line was established in April 1988.
Images：
Derivation：
The line was established in April 1988.
Clinical Data：
The patient was a smoker.
male
Caucasian
59 years
Comments：
The line was established in April 1988.
The patient was a smoker.
40 pack years.
Complete Growth Medium： HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)
Subculturing：
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 twice weekly is recommended.
Medium renewal: Every 2 to 3 days
Cryopreservation：
RPMI 1640, 85% ; FBS, 10%; DMSO, 5%
Culture Conditions：
Temperature: 37.0°C
STR Profile：
Amelogenin: X,Y
CSF1PO: 11
D13S317: 12
D16S539: 12
D5S818: 11
D7S820: 10,12
THO1: 6
TPOX: 8,9
vWA: 17
Name of Depositor： AF Gazdar, JD Minna
Deposited As： Homo sapiens
Year of Origin： 1988
References：

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H1882 [H1882]

  • 货号: TS211318
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： NCI-H1882 H1882
商品货号： TS211318
Organism： Homo sapiens, human
Tissue： lung; Derived from metastatic site: bone marrow
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： stage E,carcinoma; small cell lung cancer
Age： 59 years
Gender： male
Ethnicity： Caucasian
Applications：
The line was established in April 1988.
Images：
Derivation：
The line was established in April 1988.
Clinical Data：
The patient was a smoker.
male
Caucasian
59 years
Comments：
The line was established in April 1988.
The patient was a smoker.
40 pack years.
Complete Growth Medium： HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)
Subculturing：
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 twice weekly is recommended.
Medium renewal: Every 2 to 3 days
Cryopreservation：
RPMI 1640, 85% ; FBS, 10%; DMSO, 5%
Culture Conditions：
Temperature: 37.0°C
STR Profile：
Amelogenin: X,Y
CSF1PO: 11
D13S317: 12
D16S539: 12
D5S818: 11
D7S820: 10,12
THO1: 6
TPOX: 8,9
vWA: 17
Name of Depositor： AF Gazdar, JD Minna
Deposited As： Homo sapiens
Year of Origin： 1988
References：

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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