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NCI-H1836 [H1836]
NCI-H1836 [H1836]
规格:
货期:
编号:TS211324
品牌:Testobio
产品名称: NCI-H1836 H1836
商品货号: TS211324
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Culture Properties: floating aggregates of round cell clusters
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage L, carcinoma; classic small cell lung cancer
Age: 52 years
Gender: male
Ethnicity: Caucasian
Karyotype: del(p11-qter)
Derivation:
The line was established in February 1988.
The patient was a smoker who received prior chemotherapy and radiation therapy.
Clinical Data:
male
Caucasian
52 years
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation with subsequent resuspension in fresh medium.
Medium Renewal: Add fresh medium as cell density increases.
Cryopreservation:
culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5%
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 11,13
D5S818: 11
D7S820: 11,12
THO1: 8,9.3
TPOX: 9,10
vWA: 16
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1988
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H1836 [H1836]

  • 货号: TS211324
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H1836 H1836
商品货号: TS211324
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Culture Properties: floating aggregates of round cell clusters
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage L, carcinoma; classic small cell lung cancer
Age: 52 years
Gender: male
Ethnicity: Caucasian
Karyotype: del(p11-qter)
Derivation:
The line was established in February 1988.
The patient was a smoker who received prior chemotherapy and radiation therapy.
Clinical Data:
male
Caucasian
52 years
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation with subsequent resuspension in fresh medium.
Medium Renewal: Add fresh medium as cell density increases.
Cryopreservation:
culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5%
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 11,13
D5S818: 11
D7S820: 11,12
THO1: 8,9.3
TPOX: 9,10
vWA: 16
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1988
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

合作单位: