你好,请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
联系电话: call_new 0574-87917803
NCI-H1793 [H1793]
NCI-H1793 [H1793]
规格:
货期:
编号:TS211335
品牌:Testobio
产品名称: NCI-H1793 H1793
商品货号: TS211335
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: adenocarcinoma; non-small cell lung cancer
Age: 52 years
Gender: female
Derivation:
The line was established in December 1987.
Clinical Data:
female
The tissue donor was a non-smoker.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Cryopreservation: Freeze medium: RPMI 1640, 85%; fetal bovine serum, 10%; DMSO, 5%.
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 13
D5S818: 11,12
D7S820: 7,8
THO1: 9,9.3
TPOX: 8
vWA: 18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1987
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

首页 > 产品中心 > 微生物培养 > 菌株 > null > NCI-H1793 [H1793]

NCI-H1793 [H1793]

  • 货号: TS211335
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H1793 H1793
商品货号: TS211335
Organism: Homo sapiens, human
Tissue: lung
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: adenocarcinoma; non-small cell lung cancer
Age: 52 years
Gender: female
Derivation:
The line was established in December 1987.
Clinical Data:
female
The tissue donor was a non-smoker.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Cryopreservation: Freeze medium: RPMI 1640, 85%; fetal bovine serum, 10%; DMSO, 5%.
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 13
D5S818: 11,12
D7S820: 7,8
THO1: 9,9.3
TPOX: 8
vWA: 18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1987
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

合作单位: