NCI-H1573 [H1573]

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货期:

编号:TS211338

品牌:Testobio

产品名称：	NCI-H1573 H1573
商品货号：	TS211338
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: soft tissue
Product Format：	frozen
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	adenocarcinoma (stage 4)
Age：	35 years
Gender：	Female
Ethnicity：	Caucasian
Derivation：	The line was established in December 1986.
Clinical Data：	35 years Caucasian female The patient received prior radiation therapy. The patient was a smoker. 15 pack years.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Cryopreservation：	Freeze medium: complete culture medium described above supplemented with 7.5% (v/v) DMSO.
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X CSF1PO: 10,12 D13S317: 11,13 D16S539: 12 D5S818: 11,13 D7S820: 9,11 THO1: 6 TPOX: 8,11 vWA: 17,18
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	1986
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H1573 [H1573]

货号: TS211338
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产品名称：	NCI-H1573 H1573
商品货号：	TS211338
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: soft tissue
Product Format：	frozen
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	adenocarcinoma (stage 4)
Age：	35 years
Gender：	Female
Ethnicity：	Caucasian
Derivation：	The line was established in December 1986.
Clinical Data：	35 years Caucasian female The patient received prior radiation therapy. The patient was a smoker. 15 pack years.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Cryopreservation：	Freeze medium: complete culture medium described above supplemented with 7.5% (v/v) DMSO.
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X CSF1PO: 10,12 D13S317: 11,13 D16S539: 12 D5S818: 11,13 D7S820: 9,11 THO1: 6 TPOX: 8,11 vWA: 17,18
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	1986
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.