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NCI-H1618 [H1618]
NCI-H1618 [H1618]
规格:
货期:
编号:TS211339
品牌:Testobio
产品名称: NCI-H1618 H1618
商品货号: TS211339
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: bone marrow
Product Format: frozen
Culture Properties: suspension, multicell aggregates
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E,carcinoma; small cell lung cancer
Age: 55 years
Gender: female
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in April 1987.
Clinical Data: Caucasian
female
The patient was a smoker.
50 pack years.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Cells can be maintained by the addition of fresh medium
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation:
Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile:
Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 11,13
D5S818: 10
D7S820: 8
THO1: 6
TPOX: 8,11
vWA: 16,17
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: April, 1987
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

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NCI-H1618 [H1618]

  • 货号: TS211339
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H1618 H1618
商品货号: TS211339
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: bone marrow
Product Format: frozen
Culture Properties: suspension, multicell aggregates
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E,carcinoma; small cell lung cancer
Age: 55 years
Gender: female
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in April 1987.
Clinical Data: Caucasian
female
The patient was a smoker.
50 pack years.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Cells can be maintained by the addition of fresh medium
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation:
Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile:
Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 11,13
D5S818: 10
D7S820: 8
THO1: 6
TPOX: 8,11
vWA: 16,17
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: April, 1987
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

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