NCI-H1623 [H1623]

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货期:

编号:TS211342

品牌:Testobio

产品名称：	NCI-H1623 H1623
商品货号：	TS211342
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: lymph node
Product Format：	frozen
Culture Properties：	adherent, single cells and loosely attached clusters
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	stage 3B, adenocarcinoma; non-small cell lung cancer
Age：	58 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was established in April 1987.
Clinical Data：	58 years Caucasian male The patient was a smoker.
Complete Growth Medium：	The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution . Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: RPMI 1640 Medium, 85%; fetal bovine serum, 10%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	April, 1987
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H1623 [H1623]

货号: TS211342
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产品名称：	NCI-H1623 H1623
商品货号：	TS211342
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: lymph node
Product Format：	frozen
Culture Properties：	adherent, single cells and loosely attached clusters
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	stage 3B, adenocarcinoma; non-small cell lung cancer
Age：	58 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was established in April 1987.
Clinical Data：	58 years Caucasian male The patient was a smoker.
Complete Growth Medium：	The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution . Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: RPMI 1640 Medium, 85%; fetal bovine serum, 10%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	April, 1987
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.