NCI-H1666 [H-1666, H1666]

规格:

货期:

编号:TS211345

品牌:Testobio

产品名称：	NCI-H1666 H-1666, H1666
商品货号：	TS211345
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Product Format：	frozen
Morphology：	epithelial (many rounded cells)
Culture Properties：	loosely adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	adenocarcinoma; bronchoalveolar carcinoma
Age：	50 years
Gender：	female
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was established in June 1987.
Clinical Data：	50 years Caucasian female The patient received prior radiation therapy. The tissue donor was a non-smoker.
Complete Growth Medium：	The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 11 D13S317: 15 D16S539: 13 D5S818: 10 D7S820: 8,11 THO1: 8,9.3 TPOX: 11 vWA: 16,17
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	June 1987
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455

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NCI-H1666 [H-1666, H1666]

货号: TS211345
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产品名称：	NCI-H1666 H-1666, H1666
商品货号：	TS211345
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Product Format：	frozen
Morphology：	epithelial (many rounded cells)
Culture Properties：	loosely adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	adenocarcinoma; bronchoalveolar carcinoma
Age：	50 years
Gender：	female
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The line was established in June 1987.
Clinical Data：	50 years Caucasian female The patient received prior radiation therapy. The tissue donor was a non-smoker.
Complete Growth Medium：	The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 11 D13S317: 15 D16S539: 13 D5S818: 10 D7S820: 8,11 THO1: 8,9.3 TPOX: 11 vWA: 16,17
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	June 1987
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455