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NCI-H1672 [H1672]
NCI-H1672 [H1672]
规格:
货期:
编号:TS211346
品牌:Testobio
产品名称: NCI-H1672 H1672
商品货号: TS211346
Organism: Homo sapiens, human
Tissue: lung
Cell Type: lymphoblast
Product Format: frozen
Culture Properties: suspension, multicell aggregates
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage L,carcinoma; classic small cell lung cancer
Age: 58 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in July 1987.
Clinical Data:
The patient was a smoker.
male
Caucasian
58 years
Comments:
A lymphoblastoid line from the same patient is available as ATCC CRL-5959.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation:
Freeze medium: Complete growth medium supplemented with an additional 15% fetal bovine serum and 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 10
THO1: 10
TPOX: 11
vWA: 17
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: July, 1987
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H1672 [H1672]

  • 货号: TS211346
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H1672 H1672
商品货号: TS211346
Organism: Homo sapiens, human
Tissue: lung
Cell Type: lymphoblast
Product Format: frozen
Culture Properties: suspension, multicell aggregates
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage L,carcinoma; classic small cell lung cancer
Age: 58 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Derivation:
The line was established in July 1987.
Clinical Data:
The patient was a smoker.
male
Caucasian
58 years
Comments:
A lymphoblastoid line from the same patient is available as ATCC CRL-5959.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation:
Freeze medium: Complete growth medium supplemented with an additional 15% fetal bovine serum and 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 10
THO1: 10
TPOX: 11
vWA: 17
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: July, 1987
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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