| 产品名称： | NCI-H1436 H1436 |
|---|---|
| 商品货号： | TS211350 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: lymph node |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; classic small cell lung cancer |
| Age： | 39 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 3p is normal |
| Clinical Data： | 3 years old
male
Caucasian
The tumor developed in a patient with a 75 packs-year history of cigarette smoking.
|
| Comments： | The tumor developed in a patient with a 75 packs-year history of cigarette smoking. |
| Complete Growth Medium： | The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete growth medium, add the following components to the base medium:xa0
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters. Medium Renewal: Add fresh medium as cell density increases. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% CO2 in airxa0 |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 13 D5S818: 11,13 D7S820: 9,11 THO1: 7,9.3 TPOX: 9,11 vWA: 17,18 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | June, 1986 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | NCI-H1436 H1436 |
|---|---|
| 商品货号： | TS211350 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; derived from metastatic site: lymph node |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; classic small cell lung cancer |
| Age： | 39 years adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 3p is normal |
| Clinical Data： | 3 years old
male
Caucasian
The tumor developed in a patient with a 75 packs-year history of cigarette smoking.
|
| Comments： | The tumor developed in a patient with a 75 packs-year history of cigarette smoking. |
| Complete Growth Medium： | The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete growth medium, add the following components to the base medium:xa0
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters. Medium Renewal: Add fresh medium as cell density increases. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% CO2 in airxa0 |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 13 D5S818: 11,13 D7S820: 9,11 THO1: 7,9.3 TPOX: 9,11 vWA: 17,18 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | June, 1986 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
