NCI-H1437 [H1437]

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编号:TS211354

品牌:Testobio

产品名称：	NCI-H1437 H1437
商品货号：	TS211354
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Product Format：	frozen
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	stage 1, adenocarcinoma; non-small cell lung cancer
Age：	60 years
Gender：	male
Ethnicity：	Caucasian
Derivation：	The cell line was established in June 1986 from pleural effusion metastasis of lung.
Clinical Data：	Caucasian male The patient was a smoker. 60 years old 70 pack years.
Comments：	A lymphoblastoid line from the same patient is available as ATCC CRL-5958.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes)xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:6 Medium Renewal: Two times weekly Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：	Culture medium, 95%; DMSO, 5%
STR Profile：	Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 11,12 D5S818: 11,14 D7S820: 8,11 THO1: 6 TPOX: 9,10 vWA: 18
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	1986
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13.

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NCI-H1437 [H1437]

货号: TS211354
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产品名称：	NCI-H1437 H1437
商品货号：	TS211354
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Product Format：	frozen
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	stage 1, adenocarcinoma; non-small cell lung cancer
Age：	60 years
Gender：	male
Ethnicity：	Caucasian
Derivation：	The cell line was established in June 1986 from pleural effusion metastasis of lung.
Clinical Data：	Caucasian male The patient was a smoker. 60 years old 70 pack years.
Comments：	A lymphoblastoid line from the same patient is available as ATCC CRL-5958.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes)xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:6 Medium Renewal: Two times weekly Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：	Culture medium, 95%; DMSO, 5%
STR Profile：	Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 11,12 D5S818: 11,14 D7S820: 8,11 THO1: 6 TPOX: 9,10 vWA: 18
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
Year of Origin：	1986
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13.