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NCI-H1238 [H1238]
NCI-H1238 [H1238]
规格:
货期:
编号:TS211358
品牌:Testobio
产品名称: NCI-H1238 H1238
商品货号: TS211358
Organism: Homo sapiens, human
Product Format: frozen
Culture Properties: suspension; some adherent cells
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E, carcinoma; small cell lung cancer (SCLC)
Age: 64 years
Gender: male
Ethnicity: White
Derivation:
The line was established in November 1985.
Clinical Data:
The patient was a smoker.
male
White
64 years
Comments:
The line was established in November 1985.
The patient was a smoker.
130 pack years.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by addition of fresh medium.
Cells grow as floating aggregates of round cell clusters.
Some cells will attach.
Cryopreservation:
Culture medium with an additional 45% fetal bovine serum, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,14
D16S539: 10
D5S818: 13
D7S820: 11
THO1: 9,9.3
TPOX: 8
vWA: 18,19
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1985
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H1238 [H1238]

  • 货号: TS211358
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H1238 H1238
商品货号: TS211358
Organism: Homo sapiens, human
Product Format: frozen
Culture Properties: suspension; some adherent cells
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E, carcinoma; small cell lung cancer (SCLC)
Age: 64 years
Gender: male
Ethnicity: White
Derivation:
The line was established in November 1985.
Clinical Data:
The patient was a smoker.
male
White
64 years
Comments:
The line was established in November 1985.
The patient was a smoker.
130 pack years.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing:
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by addition of fresh medium.
Cells grow as floating aggregates of round cell clusters.
Some cells will attach.
Cryopreservation:
Culture medium with an additional 45% fetal bovine serum, 95%; DMSO, 5%
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,14
D16S539: 10
D5S818: 13
D7S820: 11
THO1: 9,9.3
TPOX: 8
vWA: 18,19
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1985
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

合作单位: