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NCI-H1092 [H1092]
NCI-H1092 [H1092]
规格:
货期:
编号:TS211359
品牌:Testobio
产品名称: NCI-H1092 H1092
商品货号: TS211359
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: bone marrow
Product Format: frozen
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E, carcinoma; classic small cell lung cancer
Age: 67 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Karyotype: del(p12-p23)
Derivation:
The line was established in June 1985.
Clinical Data: male
Caucasian
67 years old
The patient was a smoker, 120 packs a year
Comments:
Fast growing.
Complete Growth Medium: The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete growth medium, add the following components to the base medium:xa0
  • 0.005 mg/ml Insulin (Sigma I1882)
  • 0.01 mg/ml Transferrin (Sigma T1147)
  • 30nM Sodium selenite (final conc.) (Sigma S9133)xa0
  • 10 nM Hydrocortisone (final conc.) (Sigma H0135)
  • 10 nM beta-estradiol (final conc.) (Sigma E2557)xa0xa0
  • extra 2mM L-glutamine (for final conc. of 4.5 mM) (Sigma G5783)xa0
  • 5% fetal bovine serum (ATCC 30-2020)
Subculturing: Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters.
Medium Renewal:xa0Add fresh medium as cell density increases.
Cryopreservation:
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1985
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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NCI-H1092 [H1092]

  • 货号: TS211359
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-H1092 H1092
商品货号: TS211359
Organism: Homo sapiens, human
Tissue: lung; derived from metastatic site: bone marrow
Product Format: frozen
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: stage E, carcinoma; classic small cell lung cancer
Age: 67 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Karyotype: del(p12-p23)
Derivation:
The line was established in June 1985.
Clinical Data: male
Caucasian
67 years old
The patient was a smoker, 120 packs a year
Comments:
Fast growing.
Complete Growth Medium: The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete growth medium, add the following components to the base medium:xa0
  • 0.005 mg/ml Insulin (Sigma I1882)
  • 0.01 mg/ml Transferrin (Sigma T1147)
  • 30nM Sodium selenite (final conc.) (Sigma S9133)xa0
  • 10 nM Hydrocortisone (final conc.) (Sigma H0135)
  • 10 nM beta-estradiol (final conc.) (Sigma E2557)xa0xa0
  • extra 2mM L-glutamine (for final conc. of 4.5 mM) (Sigma G5783)xa0
  • 5% fetal bovine serum (ATCC 30-2020)
Subculturing: Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters.
Medium Renewal:xa0Add fresh medium as cell density increases.
Cryopreservation:
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1985
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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