NCI-H1048 [H1048]

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货期:

编号:TS211360

品牌:Testobio

产品名称：	NCI-H1048 H1048
商品货号：	TS211360
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	carcinoma; small cell lung cancer
Gender：	female
Karyotype：	3p is normal
Images：
Derivation：	The line was established in April 1985 from pleural effusion metastasis of lung.
Clinical Data：	female The tissue donor was a non-smoker.
Complete Growth Medium：	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing：	Volumes used in this protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1.5 x 10⁴ to 2 0 x 10⁴ viable cells/cm² is recommended. Subcultivation Ratio: 1:3 to 1:8 Incubate cultures at 37°C. Subculture when cell concentration is between 9 x 10⁴ and 1.7 x 10⁵ cells/cm².
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Name of Depositor：	AF Gazdar, JD Minna
Year of Origin：	1985
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H1048 [H1048]

货号: TS211360
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产品名称：	NCI-H1048 H1048
商品货号：	TS211360
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: pleural effusion
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	carcinoma; small cell lung cancer
Gender：	female
Karyotype：	3p is normal
Images：
Derivation：	The line was established in April 1985 from pleural effusion metastasis of lung.
Clinical Data：	female The tissue donor was a non-smoker.
Complete Growth Medium：	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing：	Volumes used in this protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1.5 x 10⁴ to 2 0 x 10⁴ viable cells/cm² is recommended. Subcultivation Ratio: 1:3 to 1:8 Incubate cultures at 37°C. Subculture when cell concentration is between 9 x 10⁴ and 1.7 x 10⁵ cells/cm².
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Name of Depositor：	AF Gazdar, JD Minna
Year of Origin：	1985
References：	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.