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NCI-H1341 [H1341]
NCI-H1341 [H1341]
规格:
货期:
编号:TS211364
品牌:Testobio
产品名称: NCI-H1341 H1341
商品货号: TS211364
Organism: Homo sapiens, human
Tissue: lung; Derived from metastatic site: cervix
Product Format: frozen
Morphology: floating aggregates of round cells with a few attached cells.
Culture Properties: suspension
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: carcinoma; small cell lung cancer
Gender: female
Applications:
The line was established in February 1986.
Derivation:
The line was established in February 1986.
Clinical Data:
The tissue donor was a non-smoker.
female
Comments:
The line was established in February 1986.
The tissue donor was a non-smoker.
Complete Growth Medium: ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.02 mg/ml insulin
  • 0.01 mg/ml transferrin
  • 25 nM sodium selenite (final conc.)
  • 50 nM Hydrocortisone (final conc.)
  • 1 ng/ml Epidermal Growth Factor (do not filter)
  • 0.01 mM ethanolamine (final conc.)
  • 0.01 mM phosphorylethanolamine (final conc.)
  • 100 pM triiodothyronine (final conc.)
  • 0.5% (w/v) bovine serum albumin (final conc.)
  • 10 mM HEPES
  • 0.5 mM sodium pyruvate (final conc.)
  • extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing:
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium.
Cells grow as floating aggregates of round cells with a few attached cells.
Cryopreservation:
Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1986
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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NCI-H1341 [H1341]

  • 货号: TS211364
  • 好评
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  • 品牌 : TESTOBIO
产品名称: NCI-H1341 H1341
商品货号: TS211364
Organism: Homo sapiens, human
Tissue: lung; Derived from metastatic site: cervix
Product Format: frozen
Morphology: floating aggregates of round cells with a few attached cells.
Culture Properties: suspension
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: carcinoma; small cell lung cancer
Gender: female
Applications:
The line was established in February 1986.
Derivation:
The line was established in February 1986.
Clinical Data:
The tissue donor was a non-smoker.
female
Comments:
The line was established in February 1986.
The tissue donor was a non-smoker.
Complete Growth Medium: ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.02 mg/ml insulin
  • 0.01 mg/ml transferrin
  • 25 nM sodium selenite (final conc.)
  • 50 nM Hydrocortisone (final conc.)
  • 1 ng/ml Epidermal Growth Factor (do not filter)
  • 0.01 mM ethanolamine (final conc.)
  • 0.01 mM phosphorylethanolamine (final conc.)
  • 100 pM triiodothyronine (final conc.)
  • 0.5% (w/v) bovine serum albumin (final conc.)
  • 10 mM HEPES
  • 0.5 mM sodium pyruvate (final conc.)
  • extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing:
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium.
Cells grow as floating aggregates of round cells with a few attached cells.
Cryopreservation:
Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
Year of Origin: 1986
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

合作单位: