NCI-H1299

规格:

货期:

编号:TS211365

品牌:Testobio

产品名称：	NCI-H1299
商品货号：	TS211365
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: lymph node
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	carcinoma; non-small cell lung cancer
Age：	43 years adult
Gender：	male
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The cell line was established from a lymph node metastasis of the lung from a patient who had received prior radiation therapy.
Clinical Data：	male 43 years adult Caucasian
Genes Expressed：	These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B,The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B.
Cellular Products：	neuromedin B
Comments：	The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. They reported to be able to synthesize the peptide neuromedin B (NMB) at 0.1 pmol/mg protein, but not the gastrin releasing peptide (GRP).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm^2xa0flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin:X CSF1PO:12 D13S317:12 D16S539:12,13 D5S818:11 D7S820:10 THO1:6,9.3 TPOX:8 vWA: 16,17,18
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
References：	Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350 This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy.

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NCI-H1299

货号: TS211365
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产品名称：	NCI-H1299
商品货号：	TS211365
Organism：	Homo sapiens, human
Tissue：	lung; derived from metastatic site: lymph node
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	carcinoma; non-small cell lung cancer
Age：	43 years adult
Gender：	male
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The cell line was established from a lymph node metastasis of the lung from a patient who had received prior radiation therapy.
Clinical Data：	male 43 years adult Caucasian
Genes Expressed：	These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B,The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B.
Cellular Products：	neuromedin B
Comments：	The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. They reported to be able to synthesize the peptide neuromedin B (NMB) at 0.1 pmol/mg protein, but not the gastrin releasing peptide (GRP).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm^2xa0flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin:X CSF1PO:12 D13S317:12 D16S539:12,13 D5S818:11 D7S820:10 THO1:6,9.3 TPOX:8 vWA: 16,17,18
Name of Depositor：	AF Gazdar, JD Minna
Deposited As：	Homo sapiens
References：	Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350 This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy.