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NCI-BL1437 [BL1437]
NCI-BL1437 [BL1437]
规格:
货期:
编号:TS211378
品牌:Testobio
产品名称: NCI-BL1437 BL1437
商品货号: TS211378
Organism: Homo sapiens, human
Tissue: peripheral blood
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 2 Cells Contain Epstein Barr Virus (EBV) a Herpesvirus

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: normal
Age: 60 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Comments:
A lung adenocarcinoma cell line from this patient is available as ATCC CRL-5872.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 to 5 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Every two to three days (depending on cell density).
Cryopreservation:
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 11,14
D7S820: 8,11
THO1: 6,8
TPOX: 9,10
vWA: 17,18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

首页 > 产品中心 > 微生物培养 > 菌株 > null > NCI-BL1437 [BL1437]

NCI-BL1437 [BL1437]

  • 货号: TS211378
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: NCI-BL1437 BL1437
商品货号: TS211378
Organism: Homo sapiens, human
Tissue: peripheral blood
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 2 Cells Contain Epstein Barr Virus (EBV) a Herpesvirus

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: normal
Age: 60 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Comments:
A lung adenocarcinoma cell line from this patient is available as ATCC CRL-5872.
Complete Growth Medium: HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 to 5 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Every two to three days (depending on cell density).
Cryopreservation:
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 11,14
D7S820: 8,11
THO1: 6,8
TPOX: 9,10
vWA: 17,18
Name of Depositor: AF Gazdar, JD Minna
Deposited As: Homo sapiens
References:

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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