| 产品名称： | NBT-II Nara Bladder Tumor No. 2 |
|---|---|
| 商品货号： | TS211393 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | urinary bladder |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | tumor |
| Gender： | male |
| Strain： | Wistar |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was derived from a tumor which was induced with N-butyl-N-(4-hydroxybutyl) nitrosamine. |
| Comments： | NBT-II cells differentiate in vitro giving rise to tumor giant cells. The differentiation is inhibited by vitamin A. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994 |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | J Leighton, R Tchao |
| Deposited As： | Rattus sp. |
| References： | Toyoshima K, et al. Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(- 4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II. J. Natl. Cancer Inst. 47: 979-985, 1971. PubMed: 4941696 Geisinger KR, et al. Appearance of giant cells as a morphological response of rat bladder carcinoma cell line (Nara Bladder Tumor No. 2) to a continuous thermal gradient in tissue culture. Cancer Res. 38: 1223-1230, 1978. PubMed: 639055 Toyoshima K, Leighton J. Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture. Cancer Res. 35: 1873-1879, 1975. PubMed: 1093679 Tchao R. Novel forms of epithelial cell motility on collagen and on glass surfaces. Cell Motil. 2: 333-341, 1982. PubMed: 7151140 |
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| 产品名称： | NBT-II Nara Bladder Tumor No. 2 |
|---|---|
| 商品货号： | TS211393 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | urinary bladder |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | tumor |
| Gender： | male |
| Strain： | Wistar |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was derived from a tumor which was induced with N-butyl-N-(4-hydroxybutyl) nitrosamine. |
| Comments： | NBT-II cells differentiate in vitro giving rise to tumor giant cells. The differentiation is inhibited by vitamin A. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994 |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | J Leighton, R Tchao |
| Deposited As： | Rattus sp. |
| References： | Toyoshima K, et al. Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(- 4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II. J. Natl. Cancer Inst. 47: 979-985, 1971. PubMed: 4941696 Geisinger KR, et al. Appearance of giant cells as a morphological response of rat bladder carcinoma cell line (Nara Bladder Tumor No. 2) to a continuous thermal gradient in tissue culture. Cancer Res. 38: 1223-1230, 1978. PubMed: 639055 Toyoshima K, Leighton J. Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture. Cancer Res. 35: 1873-1879, 1975. PubMed: 1093679 Tchao R. Novel forms of epithelial cell motility on collagen and on glass surfaces. Cell Motil. 2: 333-341, 1982. PubMed: 7151140 |
